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內容簡介

　　《基因剋隆和DNA分析（第7版 影印版）》的英文原版是一本在國外很有影響，且使用廣泛的基因剋隆和DNA分析的入門教材。全書深入淺齣地闡述瞭分子生物學基本技術如基因剋隆、基因錶達、PCR、基因組學等的原理，同時又生動地介紹瞭基因剋隆和DNA分析在基礎研究、醫學、農學、法醫學等領域中的實際應用。
　　《基因剋隆和DNA分析（第7版 影印版）》適閤作為高等院校生物學、農林、醫藥類專業的教學參考書，也可供生命科學相關科研人員和中學生物教師參考。

內頁插圖

目錄

Preface to the Seventh Edition
About the Companion Website
Part 1 The Basic Principles of Gene Cloning and DNA Analysis
1 Why Gene Cloning and DNA Analysis are Important
1．1 The early development of genetics
1．2 The advent of gene cloning and the polymerase chain reaction
1．3 What is gene cloning?
1．4 What is PCR?
1．5 Why gene cloning and PCR are so important
1．5．1 Obtaining a pure sample of a gene by cloning
1．5．2 PCR can also be used to purify a gene
1．6 How to find your way through this book
Further reading 12

2 Vectors for Gene Cloning： Plasmids and Bacteriophages
2．1 Plasmids
2．1．1 Size and copy number
2．1．2 Conjugation and compatibility
2．1．3 Plasmid classification
2．1．4 Plasmids in organisms other than bacteria
2．2 Bacteriophages
2．2．1 The phage infection cycle
2．2．2 Lysogenic phages
Gene organization in the □ DNA molecule
The linear and circular forms of □ DNA
M13-a filamentous phage
2．2．3 Viruses as cloning vectors for other organisms
Further reading

3 Purification of DNA from Living Cells
3．1 Preparation of total cell DNA
3．1．1 Growing and harvesting a bacterial culture
3．1．2 Preparation of a cell extract
3．1．3 Purification of DNA from a cell extract
Removing contaminants by organic extraction and enzyme digestion
Using ion-exchange chromatography to purify DNA from a cell extract
Using silica to purify DNA from a cell extract
3．1．4 Concentration of DNA samples
3．1．5 Measurement of DNA concentration
3．1．6 Other methods for the preparation of total cell DNA
3．2 Preparation of plasmid DNA
3．2．1 Separation on the basis of size
3．2．2 Separation on the basis of conformation
Alkaline denaturation
Ethidium bromide-caesium chloride density gradient centrifugation
3．2．3 Plasmid amplification
3．3 Preparation of bacteriophage DNA
3．3．1 Growth of cultures to obtain a high □ titre
3．3．2 Preparation of non-lysogenic □ phages
3．3．3 Collection of phages from an infected culture
3．3．4 Purification of DNA from □ phage particles
3．3．5 Purification of M13 DNA causes few problems
Further reading

4 Manipulation of Purified DNA
4．1 The range of DNA manipulative enzymes
4．1．1 Nucleases
4．1．2 Ligases
4．1．3 Polymerases
4．1．4 DNA-modifying enzymes
4．2 Enzymes for cutting DNA： Restriction endonucleases
4．2．1 The discovery and function of restriction endonucleases
4．2．2 Type II restriction endonucleases cut DNA at specific nucleotide sequences
4．2．3 Blunt ends and sticky ends
4．2．4 The frequency of recognition sequences in a DNA molecule
4．2．5 Performing a restriction digest in the laboratory
4．2．6 Analysing the result of restriction endonuclease cleavage
Separation of molecules by gel electrophoresis
Visualizing DNA molecules in an agarose gel
4．2．7 Estimation of the sizes of DNA molecules
4．2．8 Mapping the positions of different restriction sites in a DNA molecule
Part Ⅱ The Applications of Gene Cloning and DNA Analysis in Research
Part Ⅲ The Applications of Gene Cloning and DNA Analysis in Biotechnology
Glossary
Index
基因剋隆和DNA分析（第7版 影印版） [Gene Cloning & DNA Analysis An Introduction] 下載 mobi epub pdf txt 電子書

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